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Conception and injection of a morpholino in the zebrafish egg: phenotypic analysis

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This training will provide you the knowledge you need to design a gene inactivation experiment: the design of a morpholino and the practice of injecting into the cell.

If you want to know more about this course, do not hesitate to contact us.

 

Objectives:

To acquire the theoretical and practical bases necessary for the use of morpholinos in zebrafish.

 

Location: Montpellier

Duration: 1,5 day (10,5 hours of training)

 

Our next dates:

7 & 8 December 2020

Registration deadline: 15 days before the session

 

Scientific expert: Pierre-Olivier ANGRAND

 

Program 

Theory :

Theoretical reminders

Interest of using morpholinos

Practice :

Selection of genetic sequences

Preparation of the injection station

Injection into the cell

Phenotype analysis of morphants.

 

Prerequisites: Basic notions about the zebrafish embryo

Audience: Researchers, veterinaries, engineers, technicians, PhD students and post-docs

 

Number of participants: 5 to 8 learners

Academic rate: 1200 €

Private rate: 1400 €

VTA not applicable

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