
Conception and injection of a morpholino in the zebrafish egg: phenotypic analysis

This training will provide you the knowledge you need to design a gene inactivation experiment: the design of a morpholino and the practice of injecting into the cell.
If you want to know more about this course, do not hesitate to contact us.
Objectives:
To acquire the theoretical and practical bases necessary for the use of morpholinos in zebrafish.
Location: Montpellier
Duration: 1,5 day (10,5 hours of training)
Our next dates:
7 & 8 December 2020
Registration deadline: 15 days before the session
Scientific expert: Pierre-Olivier ANGRAND
Program
Theory :
Theoretical reminders
Interest of using morpholinos
Practice :
Selection of genetic sequences
Preparation of the injection station
Injection into the cell
Phenotype analysis of morphants.
Prerequisites: Basic notions about the zebrafish embryo
Audience: Researchers, veterinaries, engineers, technicians, PhD students and post-docs
Number of participants: 5 to 8 learners
Academic rate: 1200 €
Private rate: 1400 €
VTA not applicable